On 9 August 2023, eleven days before the 2023 Ecuadorian general election, presidential candidate Fernando Villavicencio was shot and killed after leaving a Movimiento Construye campaign rally at the Colegio Anderson in Quito. As he entered a vehicle surrounded by security guards, he was shot in the head three times and was pronounced dead at a nearby clinic. One assailant, later identified as Johan David Castillo López, died in a shootout at the scene. Nine other people were injured, including legislative candidate Gisella Molina and two police officers. An autopsy listed the cause of death as cranial trauma, hemorrhaging and cerebral laceration with a cranial fracture. Villavicencio was the first Ecuadorian presidential candidate assassinated while campaigning since 1978.
Villavicencio had been a member of Ecuador's National Assembly before its dissolution and was polling among the leading presidential candidates. He had previously reported an assassination attempt in September 2022 and had received multiple death threats, including one attributed to the Sinaloa Cartel. His killing occurred amid a wider rise in gang-related political violence in Ecuador, including the assassination of Manta mayor Agustín Intriago a month earlier and the killings of two local candidates in early 2023.
In the hours after the attack, six suspects were arrested and firearms and grenades were seized; police identified them as Colombian hitmen. A video purporting to show the gang Los Lobos claiming responsibility circulated online, though its authenticity was disputed, and a later video showed people claiming to be Los Lobos members denying involvement. Additional suspects were detained in September 2023, including gang leader Laura Dayanara Castillo Velin. In October 2023, several suspects were killed inside prisons in Guayaquil and Quito.
In February 2024, prosecutors said the assassination was ordered by Los Lobos leader Carlos Edwin Angulo Lara, alias "Invisible," from prison, who directed Castillo López to carry out the killing while Castillo Velin supplied weapons and logistics. On 12 July 2024, a Quito court sentenced Angulo and Castillo to 34 years' imprisonment for ordering the assassination, with three other accomplices sentenced to 12 years. Prosecutors stated they had established the timeline of the assassination but had not yet determined the identities or motives of its ultimate masterminds.
On 3 September 2025, prosecutors charged former minister Jose Serrano, former lawmaker Ronny Aleaga, and businessman Xavier Jordan as alleged "intellectual authors" of the killing. In November 2025, a prosecution witness testified that the murder had been ordered by drug trafficker Leandro Norero, alleging that journalist Christian Zurita had solicited money from Norero to suppress reporting on an investigation, and that Norero ordered Villavicencio's killing after further publication of the material. In March 2026, a suspected Los Lobos leader was arrested in Mexico and extradited to Colombia in connection with the case.
The assassination prompted a state of emergency, military deployment, and disruption to the ongoing election, in which Villavicencio's replacement candidate, Christian Zurita, finished third.